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. 2017 Jan 5;8(7):12052–12066. doi: 10.18632/oncotarget.14511

Figure 1. Relative Pax-5 expression in breast cancer cell lines.

Figure 1

Pax-5 gene expression was assessed in a variety of commonly used breast cancer cell lines. A. Western blots were performed on total cell lysates and immuno-probed with anti-Pax-5 or anti-actin antibodies. B. Relative Pax-5 transcriptional expression levels were determined by RT-qPCR and standardized against the HPRT housekeeping gene in each respective cell model. Control samples include Pax-5 bearing B lymphocytes (Reh and Nalm6) and Pax-5 negative cells (HEK293) which were either non-transfected (NT) or transfected with recombinant Pax-5 or the empty vector alone (pcDNA). AC indicates adenocarcinoma; IDC, invasive ductal carcinoma; and NC, non-cancerous. Standard deviations are representative of triplicates from three distinct biological samples.