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. 2017 Jan 5;8(7):12052–12066. doi: 10.18632/oncotarget.14511

Figure 3. Pax-5 attenuates breast cancer cell growth.

Figure 3

MCF7 A. and MB231 B. cells were transfected with either recombinant Pax-5 or the empty vector (pcDNA) and monitored for cell growth relative to parental cells over time (1 to 4 days) using a florescent viability assay (CellTiter-Blue, Promega). GAPDH and recombinant Pax-5 expression were confirmed by Western blots (top panels) for the respective breast cancer models. C. Using Western blot, MCF7 and MB231 cells transfected with Pax-5 or pcDNA were evaluated for the expression and phosphorylation (P) profiles of extracellular-regulated kinases 1/2 (ERK1/2) and AKT (protein kinase B). Immunoblotting of Pax-5 and GAPDH were also performed as control samples. D. Results were validated using blot density quantification with pixel density values from phosphorylated forms of ERK and AKT in relation to total ERK and AKT expression levels respectively. The presented data is the calculated mean of three independent samples and is representative of three different experiments (* p<0.01).