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. 2017 Jan 5;8(7):12052–12066. doi: 10.18632/oncotarget.14511

Figure 4. Pax-5 increases breast cancer cell adherent properties.

Figure 4

A. MCF7 cells were transfected with either Pax-5 or the empty vector (pcDNA) and incubated in 96 well microplates pre-coated fibronectin (FN) or bovine serum albumin (BSA); washed; and then analysed using the CellTiter-Blue method (Promega). The presented data is the calculated mean of three independent experiments where statistical analysis by t-test indicates significant differences in respect to control parental cells (* p<0.01). B. Transfected MCF7 cells were analysed for integrin alpha-5 (Itgα5) (left panel) and Pax-5 (right panel) transactivation potential through reporter gene assays (Dual luciferase/Promega) using Itgα5-luc and CD19-luc constructs respectively. Normalization of Firefly luciferase activity in relative light units (RLU) was performed using non-inducible Renilla luciferase and presented as the calculated mean of three independent experiments where statistical analysis was determined by t-test in respect to control parental cells (* p<0.01). C. Transfected MCF7 cells were also evaluated for the expression of epithelial tight junction protein zonula occludens-1 (ZO-1) by Western blot where GAPDH was used as a control.