Figure 7. Pax-5 suppresses epithelial to mesenchymal phenotypic transitioning processes.

Transfected MCF7 cells with either the empty pcDNA (Ctrl) or Pax-5 were transiently induced for epithelial to mesenchymal transitioning (EMT) with TGF-β and TNF-α (10 ng/ml each) treatments for 48 hours. Cells were then monitored for EMT markers and E-cadherin regulators using RT-qPCR. The presented data is the calculated mean of three independent experiments where statistical analysis was determined by t-test in respect to control cells (* p<0.01).