Figure 4. Nude rat bone marrow derived macrophages efficiently phagocytose P3 and P13 GBM tumor cells in an in vitro assay.

A. Representative images of derived macrophages after incubation (2 h) with CFSE (green) labeled P3 and P13 tumor cells treated with anti-CD47 antibody or controls (PBS or IgG). Co-cultures were rinsed to remove free CFSE labeled P3 cells before imaging. B. Bar chart displaying the phagocytic index (number of phagocytosed tumor cells per 100 macrophages) with anti-CD47 antibody and controls (IgG and PBS) for two different GBM derived CD47+ xenografts, P3 and P13. Error bars represent SD.