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. 2017 Jan 6;8(7):12158–12173. doi: 10.18632/oncotarget.14555

Figure 6. c-MET as a gene target of miR-23b.

Figure 6

A. SiHa cells were transiently transfected with miR-23b precursor vector (miR23b) or anti-miR-23b inhibitor (miR23bi), respectively, for 24 h. The cells were transfected with empty vector or anti-miR-negative control in parallel. c-MET mRNA expression level was determined by quantitative RT-PCR analysis. B. SiHa cells were transiently transfected with pMIR-REPORT/cMET3’UTR together with miR-23b precursor vector (miR23b) or anti-miR-23b inhibitor (miR23bi) for 24 h followed by dual-luciferase reporter assay. The cells were co-transfected with empty vector or anti-miR-negative control in parallel. Results were average from at least three separate experiments. Mean ± SD. *p<0.05, **p<0.01.