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. 2017 Jan 14;8(7):12211–12224. doi: 10.18632/oncotarget.14661

Figure 4. CAV1 phosphorylates JNK and regulates nuclear Fox3a translocation.

Figure 4

A. Representative western blots of A549 cells No treated control and 60 μg/mL of cordycepin alone or in combination with DMSO or the JNK inhibitor 10 mM of SP600125 B. Relative band intensities of Foxo3a, p-Foxo3a, cytosolic Foxo3a, and nuclear Foxo3a. C. Western blots of CAV1, p-JNK, JNK, Foxo3a, p-Foxo3a, cytosolic Foxo3a, nuclear Foxo3a. A549 cells were incubated with si-CAV1 or negative control siRNA for 48 h, transfected with a CAV1-overexpression construct for 48 h, or treated with SP600125 for 48 h. D. Relative band intensities of p-JNK, p-Foxo3a, cytosolic Foxo3a, nuclear Foxo3a, CAV1, Bax. E. A549 cells were grown on glass coverslips and treated with cordycepin or cordycepin with 10 mM of SP600125 for 48 h. Samples were analyzed by indirect immunofluorescence with confocal microscopy. Arrows indicate the nuclei. The bar indicates 50 μm.