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. 2017 Jan 17;8(7):12234–12246. doi: 10.18632/oncotarget.14685

Figure 6. Knockdown of Fn14 expression concurrent with TMZ treatment significantly increases animal survival.

Figure 6

A. Kaplan-Meier survival curves of athymic nude mice with intracranial xenografts of GBM44 cells transduced with a control shRNA or shRNA targeting Fn14 and treated with/without TMZ(control shRNA + TMZ vs. Fn14 shRNA + TMZ p<0.0008). B. a, c, e, g = control shRNA; b, d, f, h = Fn14 shRNA. a, b) H&E staining of GBM44 PDX tumor (T) and normal (N) brain tissue. Bar=300 uM. c, d) pH2AX staining of vehicle-treated brain tissue.e, f) pH2AX staining of TMZ-treated brain tissue. g, h) Cleaved Caspase 3 staining of TMZ-treated tumor tissue. Arrows highlight apoptotic cells. Bars for c-h=100 uM. C. Quantification of γH2AX positive nuclei in tumors expressing a control shRNA or Fn14 shRNA and treated with the vehicle alone or TMZ. D. Quantification of cleaved caspase 3 positive nuclei in control shRNA or Fn14 shRNA tumors treated with TMZ.