| Stem cell marker |
C-Myc |
F: CTTCTCTGAAAGGCTCTCR: TGCTGGTAGAAGTTCTCC |
62 |
182 |
| OCT-4 |
F: GCTCTGCAGCTTAGCTTCAAR: GTTGTGCATAGTCGCTGCTT |
59 |
319 |
| Nanog |
F: CTTCTGCTGAGATGCCTCR: GACCGGGACCTTGTCTTC |
59 |
158 |
| Klf-4 |
F: ACTTTGGGGTTCAGGTGCR: CGAACGTGGAGAAAGATG |
60 |
133 |
| Sox-2 |
F: GGAGCTTTGCAGGCCGTTTGR: GGAAAGTTGGGATCGAACAA |
64.5 |
460 |
| Piwil2 |
F: AGCATGAGGTTCGGCATGTTR: ARGGCATGCATGACATCCAG |
60 |
428 |
| Endoderm |
AFP |
F: AATGCTTCCAAACAAAGGR: TATGGCTTGGAAAGTTCG |
59 |
116 |
| GATA6 |
F: GACGTGAGCATGTACCCTAGCR: GCGTAGCCATTCCAGTCCT |
61 |
210 |
| Ectoderm |
Nestin |
F: TTGCCTGCTACCCTTGAGACR: GGGCTCTGATCTCTGCATCTAC |
61.5 |
145 |
| Tubb3 |
F: TCTTCTCACAAGTACGTGCCTR: CCCCACTCTGACCAAAGATGAA |
58 |
127 |
| Mesoderm |
ACTA2 |
F: CAGGGCTGTTTTCCCATCCATR: ACGTAGCTGTCTTTTTGTCCC |
62 |
81 |
| Brachyury |
F: CTATTCTGACAACTCACCTGCATR: ACAGGCTGGGGTACTGACT |
60 |
146 |
| GAPDH |
F: GTCAGTGGTGGACCTGACCTR: CACCACCCTGTTGCTGTAGC |
61 |
256 |
