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Morphology of survival long-term bone marrow cells. (a) Long-term bone marrow cells were cultured 7 days. (b) After a 1-week culture, 50μg/ml of hygromycin was added in order to select for stably transfected cells. After 1 week of selection, these cells were cultured 2 weeks in long-term culture medium. Cells observed in controls or in long-term culture in mice injected with the conjugate were viable (original magnification ×400).
