Table 3.
Frequencies of colonies in bone marrow following transfection anti-CD117-pIL3 conjugate
| Days | Control serum | Unconjugate | Control conjugate | Conjugate | ||||
| mean | sd | mean | sd | mean | sd | mean | sd | |
| 5 | 191 | 10 | 183 | 8 | 192 | 10 | 185 | 25 |
| 7 | 157 | 55 | 152 | 50 | 192 | 12 | 197 | 16 |
| 10 | 187 | 23 | 173 | 11 | 182 | 22 | 187 | 26 |
Number of colonies was measured 5, 7 and 10 days following in vivo transfection with 100μg of anti-CD117-pIL3 conjugate. Control groups corresponded to mice injected with unconjugated pIL3 and anti-CD117 mAb or with the control conjugate (G250-pIL3). 5 × 105 cells from bone marrow were cultured in complete methylcellulose. Colony (aggregates of more than 40 cells) numbers were evaluated under inverted light microscope. The data are representative of three independent experiments and are the mean of triplicate determinations ± S.D.