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. 2017 Mar 17;10:67. doi: 10.3389/fnmol.2017.00067

FIGURE 5.

FIGURE 5

Validation of rat brain RNA seq data using different controls. Some genes were selected for validation of RNA seq data by qRT-PCR. (A) RNA sequencing results (log 10 FPKM values) showing DEGs involved in sleep and neuronal regulation like OXT, RMRP, LNPEP, AVP, HIST2H4, GRIN2B, PMCH and HCRT were down regulated upon REMSD. Other set of genes up regulated upon REMSD, which includes DBH, NPAS4, EGR1 and SYT2. For validation of these RNA seq results, we have included other additional controls like large platform control (Lrg Plat), recovery control (Rec Ctrl) and Prazosin treatment group (PRZ treated). (B–M) Shows qRT-PCR results for above genes in free moving control (Free Mov), large platform control (Lrg Ctrl), REMSD, recovery control (Rec Ctrl), Prazosin treatment group (PRZ treated) (n = 5 per group). Statistical analyses were performed using one-way ANOVA followed by Newman–Keuls multiple comparison tests. Data are expressed as the mean ± SEM (∗P < 0.05, ∗∗P < 0.005, ∗∗∗P < 0.001).