Table 2.

Sequencing raw data generated and data analysis results.

Sample name	Raw reads (paired end)	Base (Gb)	GC (%)	% of data ≥ Q30	Raw read length (bp)
A) Fastq File Summary:
REMSD	15,673,204	3.1	49.67	90.6	100 x 2
Free Mov	29,657,987	5.9	48.8	88.4	100 x 2
B) Read alignment summary:
Sample name	Total reads	Aligned read count	Aligned %
REMSD	31,346,408	25,591,827	91.36
Free Mov	59,315,974	45,404,529	91.68

(A) Fastq file summary after RNA seq data generation, about 90% data passes Phred score ≥ Q30 and (B) read alignment results after data analysis, around 91% of data aligned to Rattus norvegicus genome for both REMSD and Free moving control (Free Mov).