Figure 2.

Phenotypic analysis of A. fumigatus ATCC46645, ΔmirC, ΔmirC^C2, and ΔmirC^GFP strains. The radial growth was measured in A. fumigatus WT and deletion strains grown on MM plates under (A) iron sufficiency, (B) iron starvation (400 μM BPS), (C) iron excess (10 mM FeSO₄), (D) 1 mM hydrogen peroxide, and (E) 2 mM hydrogen peroxide after 48 h at 37°C. Radial growth of ΔmirC was significantly decreased in response to iron starvation (p < 0.01) and oxidative stress at 2 mM hydrogen peroxide (p < 0.001). ΔmirC also displayed reduced conidiation compared to the WT and complemented strains when exposed to BPS (400 μM). (F) A. fumigatus WT, ΔmirC, ΔmirC^C2, and ΔmirC^GFP grown on MM plates containing iron-replete (Fe+), H₂O₂ (2mM), BPS (400 μM), and iron-excess (Fe++) for 48 h at 37°C. ΔmirC was significantly more sensitive to H₂O₂ compared to the WT and complemented strains. ΔmirC also has decreased conidiation compared to the WT and complemented strain when exposed to BPS (400 μM). ^**p < 0.01; ^***p < 0.001.