Table 2.

Real-time RT-PCR amplification conditions for each primer pair

Primer pair	PCR product size (bp)	Annealing temp (C)	No. of cycles	[MgCl2] (mmol/l)	Melting temp of PCR product (C)
COL3A1	522	64	40	3	91.2
BGN	131	58	40	5	86.3
SPARC	130	60	40	3	85.6
NID1	425	63	40	4	88.8
18S	187	60	35	4	86.8