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End melting temperature of the ceramide/POPC phase as a function of long-chain base length or acyl chain length. The POPC bilayers contained 10 mol % ceramide analog, and the end Tm of the ceramide-rich phase was determined from tPA-Cer anisotropy measurements. Each value is the average ± SEM; n = 2–3; LCB, long-chain base.
