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. 2017 Mar 14;112(5):976–983. doi: 10.1016/j.bpj.2017.01.016

Figure 4.

Figure 4

Effect of asymmetric analogs of ceramide on lateral segregation and gel-phase thermostability in POPC bilayers, as determined by tPA fluorescence lifetime analysis and anisotropy. The ceramide analogs had either a long-chain base of d16:1 or d20:1, or an N-linked acyl chain of 17:0 or 13:0. (A) Chemical structure of the two ceramide analogs. (B) Intensity-weighted average lifetime of tPA fluorescence. (C) Thermostability of the ceramide-rich gel phase based on tPA anisotropy measurements. The composition in (C) was 10 mol % ceramide analog in POPC. Each value is the average ± SEM; n = 3; ns, nanoseconds.