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. 2017 Mar 14;112(5):1023–1036. doi: 10.1016/j.bpj.2017.01.007

Figure 2.

Figure 2

Microstructural features of fibroblast-modified collagen matrices. (A) Second harmonic generation (SHG) imaging reveals collagen fiber morphologies in aligned and control matrices in the XY and XZ planes (scale bar, 50 μm); inset shows magnified images of regions marked with asterisks (scale bar, 20 μm). (B) Histograms of fiber orientations determined using curvelet transform and fiber extraction algorithm analysis are shown. (C) Corresponding quantification of fiber alignment for matrices aligned by distinct fibroblast lines are compared with an isotropic control (∗p < 0.0001, n ≥ 6 gels/group). (D) Quantification of pore sizes from SHG micrographs show larger XZ pore sizes than XY for both aligned and control matrices, whereas XY pore sizes in the aligned matrices were smaller than their control counterparts (∗p < 0.0001, n > 500 pores/group). (E) Fiber projection lengths on the XY plane for aligned and control tissues (∗p < 0.0001, n > 10 gels/group and >6000 individual fibers/group) are shown. Data are median with range (C), median with interquartile range (D), and mean ± SE (E).