Skip to main content
. 2017 Mar 14;112(5):1023–1036. doi: 10.1016/j.bpj.2017.01.007

Figure 3.

Figure 3

Breast carcinoma cells are acutely responsive to aligned collagen architecture in 3D. (A) Combined SHG and MPE imaging of GFP-expressing MDA-MB-231 cells in aligned collagen matrices show cellular morphology in response to anisotropic matrix organization (scale bar, 50 μm). (B) Histogram of cell orientations and (C) corresponding quantification of the degree of cell alignment (∗p < 0.0001, n ≥ 8/group) are shown. (D and E) Scatter plots display the correlation between the direction (D) and degree (E) of cell and fiber alignment. p-values for the null hypothesis that there is no correlation between the variables are noted in color-coded font (n > 25/group). Data are median ± range in (C).