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Cytotoxicity of 5-FU in HT-29 cells. Cells were exposed to different concentrations of 5-FU for different time intervals. At the end of the exposure, MTT cell viability was determined as described in materials and methods. (A) 1-day exposure, (B) 3-day exposure and (C) 5-day exposure time. Data represented are mean ± SD of three identical experiments made in three replicate.
