Table 1.
The PCR-RFLP conditions in the present study.
| SNPs | Primer sequences | Annealing temperature (°C) | Restriction enzyme | Digestion products (bp) |
|---|---|---|---|---|
| CYP1A1∗2A (rs4646903) | F: 5′ CAgTgAAgAggTgTAgCCgCT 3′ R: 5′ TAggAgTCTTgTCTCATgCCT 3′ |
62 | MspI | W (TT): 343 M (CC): 134, 209 |
|
CYP1A2∗1C (rs2069514) |
F: 5′ gCTACACATgATCgAgCTATAC 3′ R: 5′ CAggTCTCTTCACTgTAAAgTTA 3′ |
62.3 | DdeI | W (GG): 598 M (AA): 132, 466 |
|
CYP1A2∗1F (rs762551) |
F: 5′ CAACCCTgCCAATCTCAAgCAC 3′ R: 5′ CATggCTCTggTggACTTTTCA 3′ |
66.5 | ApaI | W (CC): 1012 M (AA): 299, 713 |
|
CYP2E1 (rs3813867) |
F: 5′ TCgAggAAACTTgTggACCC 3′ R: 5′ gCAAgTCATTggTTgTgCTgC 3′ |
65.1 | SfcI | W (GG): 329 M (CC): 35, 294 |
|
CYP2E1∗6 (rs6413432) |
F: 5′ CgACATgTgATggATCCAggg 3′ R: 5′ TCgTgATCgCCTgCCTCA 3′ |
64.7 | DraI | W (TT): 327 M (AA): 79, 248 |
bp: base pair; W: wild type; M: mutant type.