Dynamics of Oligodendroglial Lineage Cells after Demyelination in 2-Month-Old Mice
(A–D) Tissue sections immunostained for EYFP and PCNA at 4 (A), 7 (B), 15 (C), and 60 (D) days post lesion. Note the absence of any visible signs of lesion at 2 mpl.
(E) Graph showing the density of parenchymal OLIG2+ and SOX10+ cells after the lesion (∗p < 0.05, compared with the other time points and controls using one-way ANOVA, followed by the Scheffe post hoc analysis; error bars, SD).
(F) Graph showing the density of SEZ-derived OLIG2+ and SOX10+ cells after the lesion (the horizontal bars show significant p < 0.05 changes between time points and controls using one-way ANOVA, followed by the Scheffe post hoc analysis; error bars, SD).
(G) Representative image of the CC after immunostaining for OLIG2, EYFP, and CC1. Note the presence of EYFP+/CC1+ oligodendrocytes (arrowheads) and of EYFP+/OLIG2+/CC1− progenitors (arrows). The graph at the bottom left of the panel shows the density of parenchymal OPCs and oligodendrocytes in controls and at 7 dpl. The graph at the bottom right shows the respective information for the SEZ-derived cells of the CC. Overall numbers of sezOLIG2+ cells are significantly increased (indicated by the bracket), with both progenitors and oligodendrocytes being significantly increased (∗p < 0.05, using Student's t test; error bars, SEM).
(H) Indicative image of the CC after immunostaining for PCNA, OLIG2, and EYFP. Note the presence of EYFP+/PCNA+/OLIG2+ cycling progenitors (arrows). The graph at the bottom shows the fraction of each population of OPCs that is proliferating in control mice and after demyelination (∗p < 0.05, compared with control levels using one-way ANOVA per OPC pool; error bars, SD. scale bars, 15 μm (G, H); 200 μm (A–D). n = 6 mice per time point).