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. 2017 Feb 9;8(3):685–700. doi: 10.1016/j.stemcr.2017.01.007

Figure 7.

Figure 7

Dynamics of the Response of the SEZ after Demyelination in the CC

(A–D) Microphotographs of the SEZ in young-adult (A), aging (B) and (C), and aged (D) mice, after immunostaining for PCNA and OLIG2. Note the increased occurrence of PCNA+/OLIG2+ cells in the homeostatic SEZ in the 14-month-old mice (double-positive cells are indicated with arrowheads). Also note the increased numbers of proliferating cells in the 14 month SEZ at 7 dpl (C), as well as the lower numbers of proliferating cells in the 25 month SEZ in (D).

(E) The graph shows numbers of cells per length of the wall of the lateral ventricles in control and 7 dpl mice across different ages.

(F) Graph showing numbers of PCNA+ cells in control and 7 dpl mice across different ages.

(G) Graph showing the fraction of proliferating (PCNA+) cells that co-express OLIG2.

Note the significant increase in oligodendrogenic mitoses in the aging and aged SEZ (#p < 0.05 compared with young-adult control levels; $p < 0.05 compared with 14-month-old control levels; ∗p < 0.05 compared with control levels of the same age group; two-way ANOVA, followed by Scheffe's post hoc tests). Error bars depict SEM. Scale bars, 250 μm in (A–D). n = 6 mice per time point for young adult and 14-month-old mice, n = 4 for 25 month old mice.