FIG 4.

Neutralizing mouse antiserum targets autologous GP1. Pooled sera from female IFNAR^−/− BALB/c mice (n ≥ 3) vaccinated with JUNV-VSV, MACV-VSV, or PBS (α-JUNV, α-MACV, or normal mouse serum [NMS], respectively) were evaluated for neutralizing activity against MACV-VSV (M) or JUNV-VSV (J) (top), M/J chimera-VSV with JUNV GP1 (middle), and M/J chimera-VSV with MACV GP1 (bottom). Pseudovirions were incubated with antisera or PBS prior to application to Vero cells (dilution factors were as indicated in the inset key). Transduction is represented as the percentage of GFP-positive cells relative to cells receiving PBS treatment, as assessed by flow cytometry. Bars are representative of two or three independent experiments with the indicated SEM.