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. 2017 Mar 13;91(7):e00082-17. doi: 10.1128/JVI.00082-17

FIG 6.

FIG 6

The FACT complex promotes ALV integration. To determine the step of the life cycle that the FACT complex affects, retroviral intermediates were assayed by qPCR. (A) The FACT complex does not disrupt reverse transcription. The abundance of plus strand extension (PSE) products, a product of late reverse transcription, was measured by qPCR using primers within gag. There was no significant difference in PSE product abundance observed between SSRP1 knockout cells (SSRP1−/−) and cells expressing SSRP1 (SSRP1−/− + SSRP1) (n = 3). (B) The FACT complex specifically promotes ALV integration. The abundance of 2-LTR circles was measured by qPCR in cells expressing SSRP1 (SSRP1−/− + SSRP1) and SSRP1 knockout cells. A 10-fold increase in 2-LTR circles was detected in the SSRP1 knockout cells (n = 4; P < 0.005), indicating that depletion of the FACT complex inhibits integration.