FIG 5.
Potencies of antibodies in neutralizing 11 clinical isolates and 2 laboratory strains in ARPE-19 cells. Representative antibodies from each immunogenic site were incubated in titration with HCMV virus and then applied to ARPE-19 cells. The viral entry events were documented by immune staining of viral immediate early gene expression 24 h later. IC50s shown on the y axis were calculated using four-parameter curve fitting. Antibody designations and their classification to IR regions are marked on the x axis. CMV-HIG was included as a reference. Strain information is given in Table 2. The data shown are representative of two experiments.