FIG 4.

T1L μ2 alters the localization of SRSF2 by a microtubule-dependent mechanism. (A) L929 and AD-293 cells were transfected with T1L M1-HA for 20 h, fixed, and immunostained using antibodies against HA and SRSF2. Nuclei were counterstained with DAPI. (B) AD-293 cells were transfected as in panel A and immunostained using antibodies against HA and Son. (C) Three-dimensional reconstruction of z-stacks were generated from T1L M1-HA-transfected AD-293 cells immunostained with antibodies against HA and alpha-tubulin. (D) AD-293 cells were treated with either media alone (Mock), 10 μM nocodazole, or 10 μM paclitaxel for 6 h. The morphology of microtubules was visualized by immunostaining against α-tubulin. (E) AD-293 cells were transfected with T1L M1-HA for 20 h and treated with either media alone (Mock), 10 μM nocodazole, or 10 μM paclitaxel for 6 h. The cells were immunostained as in panel A. (F) Quantitation of panel E. Results are means ± the SEM (n = 141 to 489 cells per condition) and are representative of at least two independent experiments. All scale bars, 10 μm.