FIG 5.

T1L μ2 forms a complex with SRSF2 in the nucleus. (A) AD-293 cells were transfected with T1L M1-HA for 20 h, fixed, and subjected to a proximity ligation assay (PLA) using the indicated primary antibodies. Cells were imaged by confocal microscopy to obtain z-stack images of PLA signals (red) and nuclei (blue) representative of the total volume of the cell in the z axis. Scale bar, 10 μm. Quantitation of PLA results expressed as number of PLA dots per cell (n = 66 to 82 cells per condition) for a representative of at least three independent experiments. *, Significantly different from T3D μ2-HA (P < 0.001). (B) AD-293 cells were transfected with the indicated plasmids, whole-cell lysates were immunoprecipitated using anti-HA-conjugated agarose beads, and lysates (input) and immunoprecipitated extracts (IP) were resolved by SDS-PAGE for immunoblotting. The results are representative of at least two independent experiments. It is unclear why overexpressed SRSF2 appears as two distinct bands, but the upper band corresponds to SRSF2 migration in specifications provided with the antibody.