FIG 6.

SRSF2 is required for μ2 nuclear localization. L929 cells were transfected with the indicated siRNA for 72 h and transfected with the indicated expression plasmids for 18 h. (A) Cells were lysed, and protein fractions were resolved by SDS-PAGE for transfer and immunoblotting with the indicated antibodies. SRSF1 (negative control) or SRSF2 band intensities are expressed relative to control siRNA-transfected cells. (B) The cells were fixed and immunostained using antibodies against HA and SRSF2. Nuclei were counterstained with DAPI. Quantitated results (n = 13 to 51 cells per condition) are representative of two independent experiments. Scale bar, 20 μm.