FIG 5.

The Phe 43 cavity modulates the recognition of CRF01_AE HIV-1-infected primary CD4⁺ T cells by antibodies isolated from RV144 vaccinees. Shown are data for cell surface staining of primary CD4⁺ T cells that were either mock infected or infected with the WT or an H375S mutant of CRF01_AE transmitted-founder HIV-1 (IMC 703357) with A32-blockable MAbs isolated from RV144 vaccinees (CH29, CH38, CH40, CH51, CH52, CH54, CH77, CH80, CH81, CH89, CH91, and CH94). Shown are the MFIs obtained in at least 5 independent experiments. Error bars indicate means ± standard errors of the means. Statistical significance was tested by using an unpaired two-tailed t test (*, P < 0.05; **, P < 0.01; ****, P < 0.0001).