FIG 1.

Fluorescence microscopy of CDHR3 expression. HeLa cells were transduced with lentiviral vectors for stable expression of N-terminal FLAG-tagged Cys529 or Tyr529 CDHR3 variants. Following cell fixation (4% paraformaldehyde) cells were stained (red) with anti-FLAG antibody (rabbit monoclonal; Sigma F2555) to visualize CDHR3 surface expression. They were then permeabilized (0.1% Tween 20 and 0.05% Triton X-100 in phosphate-buffered saline [PBS]) and stained with DAPI (4′,6-diamidino-2-phenylindole; blue) and with an anti-CDHR3 antibody (green; anti-FLJ23834 mouse monoclonal; Abcam ab56549). Native HeLa cells (cervical carcinoma origin) are homozygous A/A (data not shown) but do not express a CDHR3 protein unless transduced or transfected. (Courtesy of Kelly Watters, reproduced with permission.)