Genome Exchange at MII Rescues Full-Term Development of Postovulatory Aged Oocytes
(A) Strategy for oocyte manipulation. The genome of 5-hr incubated oocytes is transferred into enucleated, 20-hr incubated oocytes (5G20C); oocytes are fertilized in vitro and allowed to develop.
(B) Preimplantation development after in vitro fertilization. The total number of 2PN oocytes and the number of experiments (in parentheses) is indicated above each column. The error bars show mean ± SEM. ns, not significant.
(C) Pups born after genome exchange (left) and fertile adults (right). The total number of transferred embryos and the number of experiments (in parentheses) is indicated.