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. 2017 Feb 23;8(3):619–633. doi: 10.1016/j.stemcr.2017.01.022

Figure 3.

Figure 3

Differentiation of HD and Isogenic Control hiPSCs Cells into Neurons

(A) Scheme of neuronal differentiation protocol.

(B–D) Neural induction results in robust expression of neural stem cell markers (B) Nestin (day 10) and (C) FOXG1 (day 15) as assessed by immunocytochemistry, and (D) PAX6, SOX1, and FOXG1 (day 15) as measured by qRT-PCR (n = 3 per clone; values for independent biological replicates shown as mean ± SEM).

(E and F) Further differentiation results in neurons expressing (E) MAP2ab, GABA, and SYP (synaptophysin) protein at day 48 and (F) MAP2AB, SYP, NKX2.1, and GAD65 mRNA at days 34 and 48 (n = 3 per clone; values for independent biological replicates shown as mean ± SEM).