Figure 3.

Knocking down the expression of EPS8 in HLA-A*110⁺ K562 cells by siRNA transient transfection. The HLA-A*1101⁺ K562 cells were transiently transfected with either control siRNA or si-h-EPS8 101–103) At (A) 24, (B) 48 and (C) 72 h post-transfection, the expression levels of EPS8 in the cells were examined using western blot analysis and quantified, as shown in graphs for the (D) 24, (E) 48 and (F) 72 h groups. β-actin was detected as a loading control. *P<0.05 and **P<0.01, compared with the NC group. The transfection effect at 48 h was more marked. EPS8, epidermal growth factor receptor pathway substrate 8; HLA, human leukocyte antigen; siRNA, small interfering RNA; si-h-EPS8 101–103; EPS8-targeting siRNA 101–103; NC, negative control.