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. 2016 Oct 27;14(6):5049–5056. doi: 10.3892/mmr.2016.5901

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Copyright: © Xiang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — miR-34a induced the apoptosis of SRA01/04. (A and B) SRA01/04 transfected with mock, mimic control, miR-34a mimic and Annexin V-FITC and PI staining for the detection of apoptosis by flow cytometry; (C) In situ analysis of apoptosis in SRA01/04 by TUNEL. Red color indicates TUNEL-positive cells. (D) Immunofluorescence microscopic analysis of active caspase 3 protein expression in SRA01/04 cells. It was indicated that SRA01/04 cells transfected with miR-34a mimic exhibited increased apoptosis compared with mock and mimic control. Magnification, ×40. Data are presented as the mean ± standard deviation; *P<0.05. miR, microRNA; FITC, fluorescein isothiocyanate; PI, propidium iodide; NS, non-significant.