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. 2016 Oct 27;14(6):5049–5056. doi: 10.3892/mmr.2016.5901

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Copyright: © Xiang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — siE2F3 induced the apoptosis of SRA01/04 cells. (A and B) SRA01/04 cells transfected with siE2F3 or siNC, and Annexin V-FITC and PI staining for the detection of apoptosis by flow cytometry. (C) Immunofluorescence microscopic analysis of active caspase 3 protein expression of SRA01/04 cells. (D) The apoptosis of SRA01/04 cells observed by the TUNEL assay. Red color indicated TUNEL-positive cells. Those results indicated that SRA01/04 transfected with miR-34a exhibited higher apoptosis compared with the mock and mimic control. Magnification, ×40. Data are presented as the mean ± standard deviation; *P<0.05. si, small interfering; NC, negative control; FITC, fluorescein isothiocyanate; PI, propidium iodide.