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. 2017 Jan 2;37(2):684–694. doi: 10.3892/or.2017.5346

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Copyright: © Okuyama et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Spirulina LPS is incapable of activating NK cells but elicits immunity against reimplanted tumors. (A) MH134 tumors from E. coli or Spirulina LPS-treated C3H/HeN mice were surgically removed on day 22, and rechallenged with MH134 tumors on day 27. Tumor growth was measured. Data represent mean ± SE of 3–5 mice per group. *P<0.05 compared with saline. (B) Freshly prepared spleen cells from C3H/HeN or C3H/HeJ mice treated with PBS, E. coli, or Spirulina LPS were co-cultured with ⁵¹Cr-labeled YAC-1 or MH134 cells. The cytolytic activity of NK cells against YAC-1 or MH134 target cells was assessed in a chromium release assay. *P<0.05 compared with PBS.