Figure 2.
TCL1 Enhances Reprogramming as a Downstream Target of KLF4
(A) Cdh1 and Rex1 mRNA levels in MEFs reprogrammed for ∼30 days by SeVdp(fK-OSM). Two days prior to the reprogramming, MEFs were infected with a retrovirus expressing each candidate gene and selected with G418. Data represent means ± SEM of three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.005 versus iPSCs(Low-K).
(B) Cdh1 and Rex1 mRNA levels in paused iPSC(Low-K) clones that express each candidate gene after ∼30 days of retrovirus infection. MEFs were first reprogrammed by SeVdp(fK-OSM) without Shield1 to generate paused iPSC(Low-K) clones, which were then infected with an empty retrovirus or a retrovirus expressing each candidate gene and selected with G418. Control paused iPSC(Low-K) clones were not infected with retrovirus but treated with 100 nM Shield1 to allow them to undergo reprogramming for ∼30 days. Data represent means ± SEM of three independent experiments. ∗∗p < 0.01, ∗∗∗p < 0.005 versus paused iPSC(Low-K) clones infected with an empty retrovirus (Low-K + vector).
(C) Cdh1 and Rex1 mRNA levels in MEFs reprogrammed by SeVdp(fK-OSM) with or without the retrovirus-transduced shRNA against Tcl1 at day 30. Data represent means ± SEM of three independent experiments. ∗p < 0.05.