Inhibition of the Canonical Notch Pathway Does Not Enhance Reprogramming
(A) qPCR analysis of Notch target genes in MEFs reprogrammed by GHMT, at day 15; n = 3 biological replicates.
(B) Enrichment plots of Notch pathways gene sets, and its nominal p value.
(C–E) αMHC-GFP MEFs were infected with GHMT and an shRNA scramble (scr) or an shRNA against Rbpj-k. At day 15, Rbpj-k mRNA expression was analyzed by qPCR (C), and different cardiac markers were analyzed by qPCR (D) or by immunostaining (E); n = 3 biological replicates. Scale bar, 100 μm.
Data are presented as means ± SD. ∗∗∗p < 0.001. See also Figure S5.