Skip to main content
. 2016 Nov 29;15(1):57–64. doi: 10.3892/mmr.2016.5974

Figure 5.

Figure 5.

Western blot analysis of VEGF, MMP-2, MMP-9, occludin and collagen-IV in the ischemic cortical tissue at 24 h following reperfusion. β-actin was used as a loading control. (A) The expression of VEGF was significantly inhibited in the RB-222-treated groups compared with the MCAO and IgG groups. In the RB-222-treated groups, MMP-2 and MMP-9 levels were significantly decreased compared to the levels in the MCAO and IgG groups. (B) A significant reduction in occludin and collagen-IV protein expression levels were observed in the MCAO and IgG groups when compared with the Sham group, while RB-222 treatment markedly reversed the reduction of occludin and collagen-IV expression. Data are presented as the mean ± standard deviation (n=6). #P<0.05 vs. Sham; *P<0.05 vs. MCAO or IgG; &P<0.05 vs. RB-222 (5 µg). VEGF, vascular endothelial growth factor; MCAO, middle cerebral artery occlusion; MMP-2, metalloproteinase-2; MMP-9, metalloproteinase-9.