Figure 4.

Resveratrol treatment increases the binding of SirT1 to the TNF-α promoter in N and T cells. Results of the PCR analysis (above) and RT-qPCR analysis (below) are shown. ChIP analyses indicated that the binding of SirT1 to the TNF-α promoter increased at 2 h in the N and T cells. Data for RT-qPCR are presented as the mean ± standard error of the mean of three independent experiments and are presented as the increase in binding relative to that of N cells at 0 h (arbitrary unit of one). *P<0.05 2 h group vs 0 h group. The standard PCR results of the ChIP samples with the same primer sets as in the RT-qPCR are representative of three independent experiments. PCR, polymerase chain reaction; N, normal; T, tolerant; SirT1, sirtuin 1; TNF-α, tumor necrosis factor-α.