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PD reduces mitochondrial ROS production in isolated neurons following middle cerebral artery occlusion. (A) Intracellular ROS were detected by DCFH-DA and observed by fluorescent microscopy. Original magnification, ×400. (B) Quantification of MitoSOX Red fluorescence by flow cytometry. Data are presented as the mean + SD (n=6 in each group). *P<0.05 vs. sham group; #P<0.05 vs. vehicle group. PD, polydatin; ROS, reactive oxygen species.
