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. 2016 Dec 28;37(2):761–767. doi: 10.3892/or.2016.5337

Figure 3.

Figure 3.

Expression of ARRB1, p-BRCA1, p-ATR, p-Chk1 and GAPDH in the H520 cell line (A) transfected with siARRB1-1 or siARRB1-2 or (B) the overexpression plasmid ARRB1(+) following treatment with 1.5 µM cisplatin. The phosphorylated ATR and Chk1 were downregulated after ARRB1 knockdown; on the contrary, ATR and Chk1 maintained phosphorylation activation with ARRB1 transfection. Expression of ARRB1, γ-H2AX, p-NF-κB and GAPDH in the H520 cell line (C) transfected with the overexpression plasmid ARRB1(+) or (D) with siARRB1 following treatments with various concentrations of cisplatin. Depletion of ARRB1 inhibited the phosphorylation of H2AX, on the contrary, overexpression of ARRB1 increased the phosphorylation of H2AX.