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. 2017 Mar 2;8(3):605–618. doi: 10.1016/j.stemcr.2017.01.027

Figure 3.

Figure 3

Generation of Chimeric Mice Engrafted with FH iHeps

(A) Schematic view of derivation of LRG mice.

(B) Strategy for generation of FH chimeric mice, and assessment of LDL-C level and endothelial function.

(C and D) Immunohistochemical staining for LDLR, hNA, and hALB in LRG mouse liver repopulated with the indicated iHeps or Matrigel (vehicle). Zoomed images for LDLR and hNA are shown. Scale bars represent 50 μm (C) and 200 μm (D).

(E and F) Whole-slide scan image (E) and snapshot images (F) of hALB staining in mouse liver repopulated with +/+ iHeps. The location of snapshot images in the whole section is shown in the corner. Scale bars represent 2 mm (E and top left image in F) and 400 μm (remaining three images in F).

(G) Scatter diagram shows the percentage of engrafted hALB+ iHeps in LRG mouse liver calculated based on whole-slide scanning images. Each dot represents one mouse with no less than four sections analyzed. Error bars indicate SEM.

(H) Bar graph shows the percentage of repopulated hNA+ iHeps in LRG mouse liver (n = 4, including sections of LRG mice engrafted with +/+ iHeps × 1, +/− iHeps × 2, and −/− iHeps × 1); quantification of hNA+ cells was performed by manual counting (see Supplemental Experimental Procedures for detailed description). Error bars indicate SEM.

(I) Plasma LDL-C levels in LRG mice engrafted with the indicated iHeps under normal diet (left panel) and HFHC diet (right panel) assessed at the indicated time points after iHep transplantation; n indicates number of mice. p Values are indicated on the figure and were obtained using a Kruskal-Wallis test; error bars indicate SEM.

(J) Percentage change of plasma LDL-C from baseline at weeks 1, 4, 8, and 12 post-transplantation in LRG mice fed with HFHC diet and engrafted with +/+ iHeps or pHH; n indicates number of mice. p Values are indicated on the figure and were obtained using a Kruskal-Wallis test; error bars indicate SEM.

(K) Immunohistochemical staining for hALB and hNA in LRG mouse liver repopulated with +/+ iHeps and pHH 12 weeks post-engraftment. Scale bars represent 200 μm.