Table I.

Primers used to evaluate the expression of osteogenic genes in MC3T3-E1 cells.

Gene	Primer sequences (5′-3′)	Amplicon (bp)
OCN	F: TGACCTCACAGATGCCAAGC; R: GCGCCGGAGTCTGTTCACTA	94
ALP	F: CCAGTGAGCAGGACACGATG; R: TGAAGGGAGCCAGTCCAAAG	108
Runx2	F: GAACTACTCCGCCGAGCTCC; R: TGAAACTCTTGCCTCGTCCG	107
OPN	F: TGGCAGTGATTTGCTTTTGC; R: GGGTGCAGGCTGTAAAGCTTC	100
OSX	F: TCGTCTGACTGCCTGCCTAGT; R: TTGCCTGGACCTGGTGAGAT	107
GAPDH	F: CGTGTTCCTACCCCCAATGT; R: TGTCATCATACTTGGCAGGTTTCT	73

F, forward; R, reverse; OCN, ostoecalcin; ALP, alkaline phosphatase; Runx2, runt-related transcription factor 2; OPN, osteopontin; OSX, osterix.