Table 1.
Effect of extraction methods (maceration and shaking) and solvent type on total phenol and total flavonoid content in different parts of Achilea eriophora and Achilea biebersteinii from Iran
| Plant organ | Solvent | Total phenolmg GAE/100 g dry plant tissue | Total flavonoidmg QUE/100 g dry plant tissue |
|---|---|---|---|
| Stem ( A. eriophora ) | methanol | 316.79 ± 43.32 | 92.13±4.02 |
| 278.02 ± 110.33 | 88.12±8.48 | ||
| ethanol | 229.93 ± 9.02 | 70.04±2.75 | |
| 225.37 ± 37.59 | 59.25±4.30 | ||
| Leaves | methanol | 1050.82 ± 184.20 | 244.06±1.27 |
| 890.17 ± 75.27 | 232.08±8.23 | ||
| ethanol | 622.76 ± 138.90 740.92 ± 84.48 |
226.42±1.71 196.58±6.71 |
|
| Aerial parts | methanol | 667.74 ± 73.30 | 216.45±2.15 |
| 581.09 ± 98.45 | 201.69±44.36 | ||
| ethanol | 327.15 ± 46.80 | 93.81±2.89 | |
| 365.29 ± 142.42 | 81.83±12.99 | ||
| Inflorescence | methanol | 812.23 ± 64.39 | 216.56±1.67 |
| 807.46 ± 105.01 | 190.13±16.35 | ||
| ethanol | 570.72 ± 51.53 | 156.97±4.88 | |
| 566.16 ± 55.75 | 133.89±10.76 | ||
| Stem ( A.biebersteinii ) | methanol | 293.36 ± 77.24 | 77.79±4.40 |
| 284.45 ± 51.04 | 74.00±4.09 | ||
| ethanol | 149.91 ± 16.39 | 74.19±7.17 | |
| 164.84 ± 12.84 | 71.01±1.54 | ||
| Leaves | methanol | 1168.98 ± 146.10 | 249.99±12.01$ |
| 1156.34 ± 76.92 | 264.00±62.16$ | ||
| ethanol | 694.48 ± 56.81 | 236.36±3.25 | |
| 719.15 ± 26.30 | 207.82±5.08 | ||
| Aerial parts | methanol | 848.30 ± 121.90 | 203.86±6.63 |
| 821.14 ± 51.98 | 210.21±21.79 | ||
| ethanol | 454.02 ± 57.88 | 214.91±4.00 | |
| 389.13 ± 81.48 | 188.15±1.93 | ||
| Inflorescence | methanol | 1657.58 ± 36.45* | 226.09±4.83 |
| 1441.79 ± 215.27 | 197.49±28.53 | ||
| ethanol | 1347.47 ± 61.37 | 184.99±8.13 | |
| 1228.68 ± 50.85 | 169.25±6.73 |
In each row, presented data belong to maceration and shaking methods, respectively.
*, $
p<0.05. Data are means ± SD of five replicates.