Table 2. Data processing parameters.
Parameter | NC particle | P4 hexamer | NC vertex |
---|---|---|---|
Particles* | 13,291 (16,466) | 56,448 (159,492) | 56,448 (159,492) |
Box size (pixels) | 512 | 128 | 128 |
Pixel size (Å) | 1.35 | 1.35 | 1.35 |
Symmetry | I1 | C6 | C1 |
Resolution (Å) | 4.0 | 9.1 | 9.1 |
B-factor (Å2)† | −177 | −600‡ | −600‡ |
NC, nucleocapsid.
*Number of particles (or subparticles in the case of P4 hexamer and NC vertex) chosen for averaging the final map is given. The number of all particles extracted from the micrographs (or all subparticles extracted from particles) is given in parenthesis.
†Inverse B-factor used for sharpening of the map.
‡Value of B-factor determined ad hoc.