Figure 4.

Workflow for the development of an optimal 3D model that is highly predictive for the behavior of a potential drug candidate in a clinical setting. The results obtained from studies in a 3D model are used to make a prediction on the outcome of in vivo studies or guide the design of improved drug candidates, and are in turn validated with data from animal and clinical studies. Knowledge of physical principles can be used to further improve the model, and the evidence obtained will in turn lead to more knowledge of physical principles that are difficult to study in animal models and patients. Technical innovations allow the incorporation of novel features, as well as provide avenues for novel or automated analysis methods that can enhance the robustness or increase the throughput of the model.