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SDS PAGE analysis of ADP-ribosylation of histone H1.2 with ARTD2, ARTD5 and ARTD 6 using NAD+ analogues 5 and 6. Upper panel shows Coomassie Blue staining; lower panel shows TMR fluorescence. Experimental details are provided in Supporting Information File 1. *High unspecific staining of H1.2 in lanes 3 and 6 results from non-catalytic bond formation of NAD+ analogues with the protein.
