Figure 5.

The effect of conditioned media from prostate cancer cell lines upon ASPN expression levels in fibroblasts. Serum-free conditioned media was prepared from PC-3 and LNCaP cells, and added to fetal prostate fibroblasts (EPF), primary prostate stromal cells (PrSC), and 3 isolates of CAFs for 24 h followed by qRT–PCR analysis of ASPN mRNA. Fold increase compared to control media, and normalised to TBP internal control mRNA. Error bars indicate s.e.m. CM from PC-3 cells induced an increase of ASPN mRNA but LNCaP CM did not; in EPF, PrSC and 2 of 3 CAFs. (ANOVA followed by Tukey multiple comparison, *P<0.05).